1) Why might you find that your DNA isolation might have multiple “perfect” matches (1.000) for more than one species? Given that 16S genes are used almost universally to discrimanate between species, How can you explain this result?

2) Why might you obtain a sequence trace that shows very strong signal (tall, well-isolated peaks) but that nontheless has both major and minor peaks at most nuceotide positions. What does this result indicate? Why might you fail to get any sequence match scoring above 0.7000?