U5 Application Assignmnet – Instructions’• U5: Application Assignmnet – Instructions
Web Page
Actions for ‘U5: Application Assignmnet – Instructions’

Genetic Biotechnology

Resources:
1. Textbook
2. Interactive animation on gene microarrays http://www.unsolvedmysteries.oregonstate.edu/microarray_07
3. Video on genetic engineering in bacteria (3.5 min) http://www.youtube.com/watch?v=zlqD4UWCuws
4. Interactive slide show on Polymerase Chain Reaction (PCR) DNA replication http://www.pbslearningmedia.org/asset/biot09_int_pcr/
5. Application Assignment Rubric
Instructions:
• Review the sections on transcription, translation, mutations, and DNA replication in your textbook. Enrichment information on genetic biotechnology may be found in Chapter 11.
• Engage in an interactive animation on gene microarrays
• Watch a video on genetic engineering in bacteria
• Explore an interactive slide show on PCR
• Respond to eight prompts (1-8) in a single MS word document, using complete sentences. Your response for all the eight prompts combined should be at least 200 words for full credit. Please review the Application Assignment rubric for detailed expectations as your responses will be evaluated for completeness, content, comprehension, clarity, and citation.
• Upload your responses to the appropriate area by the due date specified in the Course Schedule section of the Syllabus.
Introduction: Genetic Biotechnology
While there are still exciting details to be researched and studied about gene expression, the basic mechanics of gene expression and replication are understood well enough that we can apply this information to modern technology used in research and in daily life situations. This assignment explores some of these applied genetic biotechnologies that can/will affect your life. It is important for you to know the potential of these technologies for your health and use.

Prompts Section 1:
Many diseases are caused by cells expressing genes at too low or too high a level compared to normal cells. Medical researchers can examine samples from a patient to compare their gene expression levels of certain genes to people who do not have the disease. Comparative gene expression levels are also used in research to determine how gene expression changes under different environmental conditions. Watch the interactive animation of a microarray experiment http://www.unsolvedmysteries.oregonstate.edu/microarray_07
1.Using your own words, explain the Central Dogma flow of information in biology.
2. The microarray measures gene expression based on mRNA levels and is therefore measuring transcription. Describe in your own words what is happening during the stages of transcription.
3.How might this genetic biotechnology be important for you? Give a real or imagined example.

Prompts Section 2

Watch the 3.5 min https://www.youtube.com/watch?v=zlqD4UWCuws

The video mentions that this process “reprograms the bacteria to allow them to make insulin, just as they would make their other proteins.”
4. Describe the details of translation that have been omitted by this video. (What RNA molecules are involved? What is the protein synthesis machinery? How does the peptide chain grow?)
Sometimes, deliberate changes, or mutations, are introduced to a gene before it is given to bacteria to produce.
5. Give an example of one type of mutation in the DNA and describe how it may (or may not!) affect the resulting protein sequence.
6. How might protein production by genetic engineering biotechnology be useful to you? Give a real or imagined example.
Prompts Section 3:
Explore the interactive slide show on DNA replication by Polymerase Chain Reaction http://www.pbslearningmedia.org/asset/biot09_int_pcr/
Polymerase chain reaction technology was developed as a way to make copies of DNA without cells.
7. Relate the ingredients shown in the slide show test tube to the components involved in DNA replication. Why is each ingredient added to the PCR reaction?
PCR can be used to amplify and compare DNA sequences from species to species, or person to person. PCR can detect certain disease gene sequences, or can find DNA sequences in common between parents and children. PCR can even be performed on DNA found in skin or blood samples of unknown persons to compare to known persons.
8. How might PCR biotechnology be useful to you? Give a real or imagined example.
PLACE THIS ORDER OR A SIMIRAL ONE AND GET AN AMAZING DISCOUNT):-