have started laboratory report.
Here are list that need to be finished.

1. Title
2. Purpose
3. Results
4. Discussion
5. Conclusion
6. Supplemental Question (see attached)

I have attached 4 documents
1. Lab guideline (must follow guideline – detail needed)
2. Experiment 1 (DNA Profiling) lab procedure & Supplemental Question (end page)
3. Lab Result
4. pg1-6 All the things that I have completed so far.

Please let me know if you have any questions
Guidelines for Laboratory Notebook.
Note: although projects might be done in groups, your notebooks are worked on individually. You may discuss the project and results with others, but the notebook should be your own work!

I. Features

1. Notebook must be bound (no spiral notebooks).
2. Include a Table of Contents (save 1st two pages)
3. Pages should be numbered
4. If beginning a new project prior to completing another, it is recommended to either indicate the page number in which the project is being continued or to save a few pages-
e.g. “project continued on p. _” in order to be able to follow the project. Do not place the work of 2 different projects on the same page.
5. Write in ink

II. Format for Experiments

1. ALWAYS incorporate the date when the work was done (not just the beginning of an experiment). If an experiment is continued, each date must be included for each part.
2. Have a title for each experiment – this should be descriptive enough to know what the experiment is about. Think about it as if you were incorporating a title to a scientific paper. It should be brief, but not too vague. For example “Restriction Enzymes” would not be very telling of what the paper or experiment was about. In contrast “The use of restriction enzymes to identify retrotransposons in mammalian genomes” would allow the reader to decide whether he/she wishes to view the paper.
3. Purpose- provide a 1-2 sentence statement providing the objectives, goals, or purpose of the experiment. Include hypothesis if applicable. This requires more detail than simply repeating the title of the paper.
4. Methods – This should describe what was ACTUALLY done. If you followed a protocol, this may be cut and pasted into your lab notebook. However, I do not recommend this because you still need to restate the protocol to what was actually done. Therefore, it is recommended that you rewrite it (summarize) in a manner that is concise and would allow you or anyone else to repeat it (reproducibility is key!). Also, you need to rephrase statements to represent what was actually done. For example, rephrase the statement “place the sample tubes on ice for 30 minutes”, which is stated in the protocol but does not indicate you did it to “samples were placed on ice for 30 minutes” which precisely states what you did. Also, there may be certain test tubes you used, or you may have kept samples on ice for 20 minutes instead of 30 minutes. Be sure to include any modifications of the protocol. If a new experiment uses a previously performed protocol, you may write in your notebook as “refer to page…”. Again, be sure to include any modifications that may have been made. I repeat: The methods should be clear enough for you or someone else to repeat the experiment. Include names of strains or species names that are used. Be as specific as possible. Also, make sure concentrations of reagents are provided, e.g. 3mM MgCl2 or 1XTE buffer, etc. as simply stating 5 l of MgCl2 or buffer X would have no meaning to someone repeating the experiment.
5. Results – All results (positive or negative) should be included in the notebook. This includes photographs or copies of photographs or printouts of agarose gels. These should be well labeled!! Any calculations, spreadsheets, etc. should be included. Anticipate that an individual with some level of genetics background would be able to view your labeled result and have a reasonable understanding of what he or she is looking at.
6. Discussion – Although not typically incorporated into notebooks, this section is vital to determining your understanding of the experiment and how the results relate to the purpose. Since there are no exams in the course, this section is vital for assessment. This section should be well thought out and clearly demonstrate an understanding of the purpose of the experiment, interpretation of the results, e.g. assess the meaning of the results, and determine to what extent have they resolved the hypothesis or problem, or illustrated the phenomenon.
Again, the discussion is a critical component in assessment as no exams are included in this course. If no results were successfully generated, still explain what would have been expected to support or refute the hypothesis, possible reasons as to why no results were obtained, and offer possible modifications that could be incorporated to resolve experimental issues.
7. Conclusion – 2-3 sentences summarizing the project and the results.
8. Supplemental questions- Some experiments may include additional supplemental questions or exercises to do in order to allow further comprehension of the concepts of the experiments. Your answers should be included within this section of the notebook (unless otherwise indicated). Do not use these to replace the discussion section.

The instructor will indicate the individual projects. The final project will be subdivided into individual projects and then incorporated into one project. This will be explained in more detail later in the semester.

III. What not to do!

1. Do not “black out” any entries in your notebook, simply line them out.
2. Do not use pencil
3. Do not rip out pages
4. Do not incorporate any lecture notes into the notebook – Keep them separate.
5. Do not rewrite notes into separate notebook
6. Do not wait until after leaving class to write the methods you used
IV. Grading of Notebooks (periodically throughout the course)

1. Is the format being followed? 8 points
2. Is the notebook neat and readable? 6 points
3. Are the titles descriptive? 3 points
4. Is the purpose clear? 2 points
5. Are the methods understandable? 18 points
6. Are the results well organized
and with figures well-labeled and easy to interpret 20 points
7. Does the discussion indicate a clear understanding
of the project? 30 points
8. Are the conclusions reasonable? 5 points
9. Are the supplemental questions done and correct? 8 points
If you feel your notebook is “messy” and not understandable, then you may rewrite information in your book, but leave the original material there as well.

Explanations:
Format – all components included, up-to-date (if experiments not completed then up to the point of methods that have been done)
Neat and Readable – can read the writing, can follow experiment from beginning to end
Title – is it descriptive?
Purpose – does it clearly indicate the objective/hypothesis of the experiment? It should be more informative than the title.
Methods – is it exactly what was done? Can someone else view the methods and be able to repeat them (without being confused)?
Results – (not a discussion of results)- is it well organized? Are the figures well labeled so that someone viewing them can interpret them without rereading the methods section?
Discussion – does this indicate a CLEAR understanding of what the project was about? What was the purpose and what is your interpretation of the results and how it relates to the purpose?
Conclusion – summary of the work. Typically a couple of sentences.
Laboratory and Research Report Grading:
Final project reports will be submitted to turnitin as a tool to determine plagiarism (details later). You will have the opportunity to submit a draft yourself for assessing this.

I. Form and Readability

Division into sections (Title page, Abstract, Introduction, Materials and Methods, Results, Discussion, Literature Cited) – 5 pts
Data Presentation – Clarity/Style – 5 pts
English Composition (grammar, spelling, etc.) – 5 pts
Legibility, Neatness – 3 pts
Literature Citations – Accuracy/Completeness – 7 pts

II. Content
Abstract – concentrates the essential qualities of the study – 5 pts
Introduction – literature review and justification of objective/hypothesis – 15 pts
Materials and Methods – Clarity, can someone else reproduce experiment? – 15 pts
Results – Text Presentation and Description – 10 pts
– Graphics – Figures and Tables (form, title, etc.) – 10 pts
Discussion – Explanation of results, comparisons, conclusions – 15 pts
Literature Cited (appropriateness, correct format) – 5 pts
Additional details will be provided later in the semester.