CELLULAR PATHOLOGY

Cytological sample preparation and cell identification IN
PAP stain in cervical test.

1) ?Why are the slides taken through graduated alcohol prior to staining with
Haematoxylin?

2) Why is it important to remove excess Haematoxylin stain from the background
and cytoplasm?

3) Why are the slides taken through graduated alcohol prior to staining with the
counter stains?

4) Why is the order of the stains and counter stains important?
? H&E (Haemotoxylin and Eosin)
Histological sample staining and tissue recognition.
1) The role of haematoxylin within the staining process in appearance in any tissue sample?
2) What is the role of the eosin stain within the H&E staining process?

Cellular Pathology

Order Description
A surgeon is removing a lesion suspected to be BCC from the eyelid of a patient. Discuss the possible pathway for that sample, including frozen section, and indicate some of the techniques that may be used in reaching a final diagnosis.

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