Q1. A standard procedure in pufying tubulin is to extract tissue on ice, then put in GTP and warm the tissue extract to 37C. The warmed extract is then centrifuged at a speed insufficient to pellet average sized proteins, but sufficient to pellet large protein complexes. After centrifugation, supernant is discarded, and the solution is again warmed for a few minutes. Then centrifugation at the same speed described above is again performed. This process is repeated some times.

How would this process result in purification of tubulin?

What contaminating proteins would likely co-purify with tubulin?

Q2. How many grams of methane should be combusted to heat 1.30 of water from 24.0 to 92.0, assume as product and 100 percent efficiency in heat transfer?