Am trying to engineer pUC19 and insert the promoter and origin sequences of bacillus and yeast into it. I need you to mention about shuttle vectors, ecoli vectors (basic ones), yeast vectors, bacillus vevtors . Ecoli-bacillus shuttle vector and e.coli yeast shuttle vector and their applications. *I will be designing primers for origin and promoter sequences of bacillus and yeast vector and then I’ll amplify them..following transformation. Do include about the respective markers